FastQCFastQC Report
Mon 11 Dec 2017
129-M-ATAC-2-21_TGGGTTTC_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename129-M-ATAC-2-21_TGGGTTTC_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16712985
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT435110.2603424822076966Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG49950.024.382559120
CGTCTAG21650.022.729661
TAGGACG61150.019.7258874
CGGTGGG71700.019.161745120
CGTATCC164200.018.415186120
TCGCCGG89250.017.343239120
GTCCTAC100500.017.316851
TTGACGG26150.016.52145831
GGACGTG70850.016.4300586
CGCCGTT32900.015.318003120
GTAGGAC97700.014.9876053
CCGTACG22850.014.9659669
CTACAGT117000.014.8234394
AGGACGT90400.014.6710375
TACAGTG128200.014.6518775
ATACGCG28700.014.6370354
GTATAGG58650.014.6320451
TACGCGT28050.014.5483265
CGTGATT46550.014.43712528
GGTACGA20100.014.328485518
Produced by FastQC (version 0.11.2)