FastQCFastQC Report
Mon 11 Dec 2017
129-M-ATAC-2-21_TGGGTTTC_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename129-M-ATAC-2-21_TGGGTTTC_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16712985
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTA416790.2493809454145983No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGT218380.13066486926183443No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG20600.028.832431
TAGGACG61100.021.8947124
TTGACGG26500.019.24164431
GGACGTG72050.018.4823956
CTACGGT18450.017.55770713
AACTGCG31100.017.1659897
GTAGGAC101750.016.9208873
CTAGACG22150.016.7916563
AGGACGT91450.016.3338535
TAGACGC40300.016.2254624
TAGACCG20550.015.7636784
CGGCTAT36200.015.74306735
GTCCTAC94350.015.70611
GACGGCT34100.015.65686333
GACCGTT24150.015.6481387
AGACCGT29600.015.6040826
TACACGG25300.015.4122435
TACGGTG21150.015.31629814
CGTAATC22850.015.22697122
GTACCGT8300.015.1768086
Produced by FastQC (version 0.11.2)