FastQCFastQC Report
Mon 11 Dec 2017
129-M-ATAC-2-21_TGGGTTTC_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename129-M-ATAC-2-21_TGGGTTTC_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16699120
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT429220.25703150824714116Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAT23850.061.55814494
CGACGTA43400.046.20111592
ACGTATC42350.044.29715394
TGTCGGT27950.041.0984193
ACGCCGT36800.040.44980694
GGTCGAC50050.040.19899789
TCGACGT50850.039.16501691
CACCGTA38050.039.1209793
TAGATCG30450.038.3953988
TGGTCGA53550.037.5725188
TGATCGC31550.037.05520292
GACGTAT50950.036.95351493
GTGATCG32050.036.68932391
GTGGACG44600.035.81439690
TACGAGT20250.034.9082894
TGGACGC46500.034.204691
GTAGCCG53200.033.9852292
TCGGTAG26400.033.72833689
ATGTCGG36050.033.56099792
TCACCGT50200.032.49583492
Produced by FastQC (version 0.11.2)