FastQCFastQC Report
Mon 11 Dec 2017
129-M-ATAC-2-21_TGGGTTTC_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename129-M-ATAC-2-21_TGGGTTTC_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16699120
Sequences flagged as poor quality0
Sequence length126
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTA406550.2434559425885915No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGT211940.12691686747565142No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG21700.028.4765851
TAGGACG65550.018.9457024
TTGACGG29900.018.45792431
CTAGACG21500.017.5795843
GTCCTAC97250.017.3968221
TCGTACG6600.017.2734399
GTAGGAC104450.017.0015983
TAGACGC40550.016.5706754
CTACGGT20050.016.15648513
GACCGTT23550.016.0480527
ATACGCG28500.015.9985914
CGGCTAT38700.015.81109335
TACGCGT28850.015.8045015
TCTTACG22750.015.56023444
ACGCGTA28600.015.521396
TACACCG23700.015.4416785
TTAGACG23750.015.4089383
GGACGTG79400.015.2614496
CGTCTAA21500.015.068351
CGCATTA18350.015.03792712
Produced by FastQC (version 0.11.2)