data_5076 save_sample_conditions_one _Sample_conditions.ID 1 _Sample_conditions.Entry_ID 5076 _Sample_conditions.Sf_category sample_conditions _Sample_conditions.Sf_framecode sample_conditions_one loop_ _Sample_condition_variable.Sample_conditions_ID _Sample_condition_variable.Type _Sample_condition_variable.Val _Sample_condition_variable.Val_err _Sample_condition_variable.Val_units 1 pH 6.7 . n/a 1 temperature 303 . K stop_ save_ save_T1_relaxation_500_major_unfolded_state _Heteronucl_T1_list.Sf_ID -22 _Heteronucl_T1_list.ID 1 _Heteronucl_T1_list.Entry_ID 5076 _Heteronucl_T1_list.Sf_category heteronucl_T1_relaxation _Heteronucl_T1_list.Sf_framecode T1_relaxation_500_major_unfolded_state _Heteronucl_T1_list.Details ; T1 recorded at 11.7 Tesla ; _Heteronucl_T1_list.Sample_conditions_label $sample_conditions_one _Heteronucl_T1_list.Sample_conditions_ID 1 _Heteronucl_T1_list.Spectrometer_frequency_1H 500 _Heteronucl_T1_list.T1_coherence_type Nz _Heteronucl_T1_list.T1_val_units ms loop_ _Heteronucl_T1_software_apps.Sf_ID _Heteronucl_T1_software_apps.Heteronucl_T1_list_ID _Heteronucl_T1_software_apps.Software_label -22 1 $nLinLS stop_ loop_ _Heteronucl_T1_experiment.Heteronucl_T1_list_ID _Heteronucl_T1_experiment.Sample_ID _Heteronucl_T1_experiment.Sample_label _Heteronucl_T1_experiment.Experiment_ID _Heteronucl_T1_experiment.Experiment_label 1 1 $sample1 . ? stop_ loop_ _T1.Heteronucl_T1_list_ID _T1.Seq_ID _T1.Comp_ID _T1.Entry_atom_ID _T1.T1_val _T1.T1_val_err _T1.Entity_assembly_ID _T1.Entity_ID 1 3 LYS N 515.692 29.290 1 1 1 5 ILE N 549.484 30.301 1 1 1 6 ALA N 671.935 39.272 1 1 1 9 VAL N 625.321 36.926 1 1 1 10 LEU N 579.837 29.777 1 1 1 11 GLY N 552.510 33.449 1 1 1 19 ARG N 618.837 33.887 1 1 1 31 LYS N 610.034 32.190 1 1 1 34 VAL N 537.940 29.390 1 1 1 41 ILE N 551.146 30.428 1 1 1 42 LYS N 475.862 22.304 1 1 1 55 ASP N 485.224 25.895 1 1 1 56 GLY N 611.209 33.588 1 1 1 57 GLU N 496.520 26.054 1 1 1 58 THR N 559.519 31.551 1 1 1 60 GLU N 534.538 27.019 1 1 1 61 PHE N 533.758 28.413 1 1 1 63 VAL N 518.215 28.526 1 1 1 64 VAL N 518.793 27.059 1 1 1 65 GLU N 537.918 29.306 1 1 1 66 GLY N 591.199 33.361 1 1 1 69 GLY N 704.190 37.321 1 1 1 70 ALA N 531.213 27.478 1 1 1 71 GLU N 585.299 31.872 1 1 1 72 ALA N 595.050 32.676 1 1 1 73 ALA N 603.182 33.395 1 1 1 74 ASN N 495.442 30.043 1 1 1 79 GLY N 1008.162 53.793 1 1 stop_ _Heteronucl_T1_list.temp_mol_sys_comp_name_id_match 1 save_ save_T1_relaxation_750_major_unfolded_state _Heteronucl_T1_list.Sf_ID -23 _Heteronucl_T1_list.ID 2 _Heteronucl_T1_list.Entry_ID 5076 _Heteronucl_T1_list.Sf_category heteronucl_T1_relaxation _Heteronucl_T1_list.Sf_framecode T1_relaxation_750_major_unfolded_state _Heteronucl_T1_list.Details ; T1 at 17.6 Tesla ; _Heteronucl_T1_list.Sample_conditions_label $sample_conditions_one _Heteronucl_T1_list.Sample_conditions_ID 1 _Heteronucl_T1_list.Spectrometer_frequency_1H 750 _Heteronucl_T1_list.T1_coherence_type Nz _Heteronucl_T1_list.T1_val_units ms loop_ _Heteronucl_T1_software_apps.Sf_ID _Heteronucl_T1_software_apps.Heteronucl_T1_list_ID _Heteronucl_T1_software_apps.Software_label -23 2 $nLinLS stop_ loop_ _Heteronucl_T1_experiment.Heteronucl_T1_list_ID _Heteronucl_T1_experiment.Sample_ID _Heteronucl_T1_experiment.Sample_label _Heteronucl_T1_experiment.Experiment_ID _Heteronucl_T1_experiment.Experiment_label 2 1 $sample1 . ? stop_ loop_ _T1.Heteronucl_T1_list_ID _T1.Seq_ID _T1.Comp_ID _T1.Entry_atom_ID _T1.T1_val _T1.T1_val_err _T1.Entity_assembly_ID _T1.Entity_ID 2 3 LYS N 612.026 34.836 1 1 2 5 ILE N 610.294 33.539 1 1 2 6 ALA N 661.145 38.511 1 1 2 9 VAL N 580.784 31.985 1 1 2 10 LEU N 583.648 32.041 1 1 2 11 GLY N 528.682 26.944 1 1 2 19 ARG N 578.780 32.895 1 1 2 31 LYS N 629.362 35.081 1 1 2 34 VAL N 578.524 32.445 1 1 2 40 ALA N 489.146 27.260 1 1 2 41 ILE N 578.455 32.653 1 1 2 42 LYS N 542.073 33.861 1 1 2 55 ASP N 604.203 33.311 1 1 2 56 GLY N 620.521 35.971 1 1 2 57 GLU N 560.857 30.887 1 1 2 58 THR N 579.333 32.378 1 1 2 60 GLU N 593.187 33.309 1 1 2 61 PHE N 601.452 32.585 1 1 2 63 VAL N 610.360 34.635 1 1 2 64 VAL N 571.168 32.116 1 1 2 65 GLU N 603.392 33.682 1 1 2 66 GLY N 630.871 35.289 1 1 2 69 GLY N 580.940 32.940 1 1 2 70 ALA N 562.111 31.844 1 1 2 71 GLU N 617.461 35.775 1 1 2 72 ALA N 630.278 35.551 1 1 2 73 ALA N 634.022 36.680 1 1 2 74 ASN N 671.207 38.871 1 1 2 79 GLY N 1013.494 55.517 1 1 stop_ _Heteronucl_T1_list.temp_mol_sys_comp_name_id_match 1 save_ save_T1_relaxation_500_minor_unfolded_state _Heteronucl_T1_list.Sf_ID -24 _Heteronucl_T1_list.ID 3 _Heteronucl_T1_list.Entry_ID 5076 _Heteronucl_T1_list.Sf_category heteronucl_T1_relaxation _Heteronucl_T1_list.Sf_framecode T1_relaxation_500_minor_unfolded_state _Heteronucl_T1_list.Details ; T1 recorded at 11.7 Tesla ; _Heteronucl_T1_list.Sample_conditions_label $sample_conditions_one _Heteronucl_T1_list.Sample_conditions_ID 1 _Heteronucl_T1_list.Spectrometer_frequency_1H 500 _Heteronucl_T1_list.T1_coherence_type Nz _Heteronucl_T1_list.T1_val_units ms loop_ _Heteronucl_T1_software_apps.Sf_ID _Heteronucl_T1_software_apps.Heteronucl_T1_list_ID _Heteronucl_T1_software_apps.Software_label -24 3 $nLinLS stop_ loop_ _Heteronucl_T1_experiment.Heteronucl_T1_list_ID _Heteronucl_T1_experiment.Sample_ID _Heteronucl_T1_experiment.Sample_label _Heteronucl_T1_experiment.Experiment_ID _Heteronucl_T1_experiment.Experiment_label 3 1 $sample1 . ? stop_ loop_ _T1.Heteronucl_T1_list_ID _T1.Seq_ID _T1.Comp_ID _T1.Entry_atom_ID _T1.T1_val _T1.T1_val_err _T1.Entity_assembly_ID _T1.Entity_ID 3 42 LYS N 455.534 27.094 1 1 3 79 GLY N 803.418 37.428 1 1 stop_ _Heteronucl_T1_list.temp_mol_sys_comp_name_id_match 1 save_ save_T1_relaxation_500_extra_minor_unfolded_state _Heteronucl_T1_list.Sf_ID -25 _Heteronucl_T1_list.ID 4 _Heteronucl_T1_list.Entry_ID 5076 _Heteronucl_T1_list.Sf_category heteronucl_T1_relaxation _Heteronucl_T1_list.Sf_framecode T1_relaxation_500_extra_minor_unfolded_state _Heteronucl_T1_list.Details ; T1 recorded at 11.7 Tesla ; _Heteronucl_T1_list.Sample_conditions_label $sample_conditions_one _Heteronucl_T1_list.Sample_conditions_ID 1 _Heteronucl_T1_list.Spectrometer_frequency_1H 500 _Heteronucl_T1_list.T1_coherence_type Nz _Heteronucl_T1_list.T1_val_units ms loop_ _Heteronucl_T1_software_apps.Sf_ID _Heteronucl_T1_software_apps.Heteronucl_T1_list_ID _Heteronucl_T1_software_apps.Software_label -25 4 $nLinLS stop_ loop_ _Heteronucl_T1_experiment.Heteronucl_T1_list_ID _Heteronucl_T1_experiment.Sample_ID _Heteronucl_T1_experiment.Sample_label _Heteronucl_T1_experiment.Experiment_ID _Heteronucl_T1_experiment.Experiment_label 4 1 $sample1 . ? stop_ loop_ _T1.Heteronucl_T1_list_ID _T1.Seq_ID _T1.Comp_ID _T1.Entry_atom_ID _T1.T1_val _T1.T1_val_err _T1.Entity_assembly_ID _T1.Entity_ID 4 79 GLY N 533.242 31.262 1 1 stop_ _Heteronucl_T1_list.temp_mol_sys_comp_name_id_match 1 save_ save_T1_relaxation_750_minor_unfolded_state _Heteronucl_T1_list.Sf_ID -26 _Heteronucl_T1_list.ID 5 _Heteronucl_T1_list.Entry_ID 5076 _Heteronucl_T1_list.Sf_category heteronucl_T1_relaxation _Heteronucl_T1_list.Sf_framecode T1_relaxation_750_minor_unfolded_state _Heteronucl_T1_list.Details ; T1 at 17.6 Tesla ; _Heteronucl_T1_list.Sample_conditions_label $sample_conditions_one _Heteronucl_T1_list.Sample_conditions_ID 1 _Heteronucl_T1_list.Spectrometer_frequency_1H 750 _Heteronucl_T1_list.T1_coherence_type Nz _Heteronucl_T1_list.T1_val_units ms loop_ _Heteronucl_T1_software_apps.Sf_ID _Heteronucl_T1_software_apps.Heteronucl_T1_list_ID _Heteronucl_T1_software_apps.Software_label -26 5 $nLinLS stop_ loop_ _Heteronucl_T1_experiment.Heteronucl_T1_list_ID _Heteronucl_T1_experiment.Sample_ID _Heteronucl_T1_experiment.Sample_label _Heteronucl_T1_experiment.Experiment_ID _Heteronucl_T1_experiment.Experiment_label 5 1 $sample1 . ? stop_ loop_ _T1.Heteronucl_T1_list_ID _T1.Seq_ID _T1.Comp_ID _T1.Entry_atom_ID _T1.T1_val _T1.T1_val_err _T1.Entity_assembly_ID _T1.Entity_ID 5 42 LYS N 518.547 28.451 1 1 5 79 GLY N 1027.701 57.872 1 1 stop_ _Heteronucl_T1_list.temp_mol_sys_comp_name_id_match 1 save_ save_T1_relaxation_750_extra_minor_unfolded_state _Heteronucl_T1_list.Sf_ID -27 _Heteronucl_T1_list.ID 6 _Heteronucl_T1_list.Entry_ID 5076 _Heteronucl_T1_list.Sf_category heteronucl_T1_relaxation _Heteronucl_T1_list.Sf_framecode T1_relaxation_750_extra_minor_unfolded_state _Heteronucl_T1_list.Details ; T1 at 17.6 Tesla ; _Heteronucl_T1_list.Sample_conditions_label $sample_conditions_one _Heteronucl_T1_list.Sample_conditions_ID 1 _Heteronucl_T1_list.Spectrometer_frequency_1H 750 _Heteronucl_T1_list.T1_coherence_type Nz _Heteronucl_T1_list.T1_val_units ms loop_ _Heteronucl_T1_software_apps.Sf_ID _Heteronucl_T1_software_apps.Heteronucl_T1_list_ID _Heteronucl_T1_software_apps.Software_label -27 6 $nLinLS stop_ loop_ _Heteronucl_T1_experiment.Heteronucl_T1_list_ID _Heteronucl_T1_experiment.Sample_ID _Heteronucl_T1_experiment.Sample_label _Heteronucl_T1_experiment.Experiment_ID _Heteronucl_T1_experiment.Experiment_label 6 1 $sample1 . ? stop_ loop_ _T1.Heteronucl_T1_list_ID _T1.Seq_ID _T1.Comp_ID _T1.Entry_atom_ID _T1.T1_val _T1.T1_val_err _T1.Entity_assembly_ID _T1.Entity_ID 6 79 GLY N 720.900 34.486 1 1 stop_ _Heteronucl_T1_list.temp_mol_sys_comp_name_id_match 1 save_ save_heteronuclear_NOE_500_major_unfolded_state _Heteronucl_NOE_list.Sf_ID -28 _Heteronucl_NOE_list.ID 1 _Heteronucl_NOE_list.Entry_ID 5076 _Heteronucl_NOE_list.Sf_category heteronucl_NOE_list _Heteronucl_NOE_list.Sf_framecode heteronuclear_NOE_500_major_unfolded_state _Heteronucl_NOE_list.Details ; Heteronuclear noe defined as Intensity of presaturated experiment. Divided by Intensity of the reference spectrum, recorded at 11.7 Tesla. ; _Heteronucl_NOE_list.Sample_conditions_label $sample_conditions_one _Heteronucl_NOE_list.Sample_conditions_ID 1 _Heteronucl_NOE_list.Spectrometer_frequency_1H 500 _Heteronucl_NOE_list.Heteronuclear_NOE_val_type 'ratio peak height presat experiment and peak height reference experiment.' _Heteronucl_NOE_list.NOE_ref_val . _Heteronucl_NOE_list.NOE_ref_description . loop_ _Heteronucl_NOE_software_apps.Sf_ID _Heteronucl_NOE_software_apps.Heteronucl_NOE_list_ID _Heteronucl_NOE_software_apps.Software_label -28 1 $nLinLS stop_ loop_ _Heteronucl_NOE_experiment.Heteronucl_NOE_list_ID _Heteronucl_NOE_experiment.Sample_ID _Heteronucl_NOE_experiment.Sample_label _Heteronucl_NOE_experiment.Experiment_ID _Heteronucl_NOE_experiment.Experiment_label 1 1 $sample1 . ? stop_ loop_ _Heteronucl_NOE.ID _Heteronucl_NOE.Sf_ID _Heteronucl_NOE.Heteronucl_NOE_list_ID _Heteronucl_NOE.Seq_ID_1 _Heteronucl_NOE.Seq_ID_2 _Heteronucl_NOE.Comp_ID_1 _Heteronucl_NOE.Comp_ID_2 _Heteronucl_NOE.Heteronucl_NOE_val _Heteronucl_NOE.Heteronucl_NOE_val_err _Heteronucl_NOE.Entity_assembly_ID_1 _Heteronucl_NOE.Entity_ID_1 _Heteronucl_NOE.Entity_assembly_ID_2 _Heteronucl_NOE.Entity_ID_2 _Heteronucl_NOE.Atom_ID_1 _Heteronucl_NOE.Atom_ID_2 1 -28 1 3 3 LYS LYS 0.0889 0.03 1 1 1 1 N H 2 -28 1 5 5 ILE ILE -0.4382 0.03 1 1 1 1 N H 3 -28 1 6 6 ALA ALA -0.1728 0.03 1 1 1 1 N H 4 -28 1 9 9 VAL VAL 0.2075 0.03 1 1 1 1 N H 5 -28 1 10 10 LEU LEU 0.0729 0.03 1 1 1 1 N H 6 -28 1 11 11 GLY GLY -0.1476 0.03 1 1 1 1 N H 7 -28 1 19 19 ARG ARG -0.0976 0.03 1 1 1 1 N H 8 -28 1 31 31 LYS LYS 0.0074 0.03 1 1 1 1 N H 9 -28 1 34 34 VAL VAL 0.1539 0.03 1 1 1 1 N H 10 -28 1 40 40 ALA ALA 0.3718 0.03 1 1 1 1 N H 11 -28 1 41 41 ILE ILE 0.0994 0.03 1 1 1 1 N H 12 -28 1 55 55 ASP ASP 0.5727 0.03 1 1 1 1 N H 13 -28 1 56 56 GLY GLY 0.1186 0.03 1 1 1 1 N H 14 -28 1 57 57 GLU GLU 0.3126 0.03 1 1 1 1 N H 15 -28 1 60 60 GLU GLU 0.0791 0.03 1 1 1 1 N H 16 -28 1 61 61 PHE PHE 0.1849 0.03 1 1 1 1 N H 17 -28 1 63 63 VAL VAL 0.2176 0.03 1 1 1 1 N H 18 -28 1 64 64 VAL VAL 0.1771 0.03 1 1 1 1 N H 19 -28 1 65 65 GLU GLU 0.1874 0.03 1 1 1 1 N H 20 -28 1 66 66 GLY GLY 0.0581 0.03 1 1 1 1 N H 21 -28 1 69 69 GLY GLY -0.3110 0.03 1 1 1 1 N H 22 -28 1 70 70 ALA ALA 0.1954 0.03 1 1 1 1 N H 23 -28 1 71 71 GLU GLU -0.0356 0.03 1 1 1 1 N H 24 -28 1 72 72 ALA ALA 0.2344 0.03 1 1 1 1 N H 25 -28 1 73 73 ALA ALA 0.0110 0.03 1 1 1 1 N H 26 -28 1 74 74 ASN ASN 0.5671 0.03 1 1 1 1 N H 27 -28 1 79 79 GLY GLY -0.8584 0.03 1 1 1 1 N H stop_ save_ save_heteronuclear_NOE_500_minor_unfolded_state _Heteronucl_NOE_list.Sf_ID -29 _Heteronucl_NOE_list.ID 2 _Heteronucl_NOE_list.Entry_ID 5076 _Heteronucl_NOE_list.Sf_category heteronucl_NOE_list _Heteronucl_NOE_list.Sf_framecode heteronuclear_NOE_500_minor_unfolded_state _Heteronucl_NOE_list.Details ; Heteronuclear noe defined as Intensity of presaturated experiment. Divided by Intensity of the reference spectrum, recorded at 11.7 Tesla. ; _Heteronucl_NOE_list.Sample_conditions_label $sample_conditions_one _Heteronucl_NOE_list.Sample_conditions_ID 1 _Heteronucl_NOE_list.Spectrometer_frequency_1H 500 _Heteronucl_NOE_list.Heteronuclear_NOE_val_type 'ratio peak height presat experiment and peak height reference experiment.' _Heteronucl_NOE_list.NOE_ref_val . _Heteronucl_NOE_list.NOE_ref_description 'not used' loop_ _Heteronucl_NOE_software_apps.Sf_ID _Heteronucl_NOE_software_apps.Heteronucl_NOE_list_ID _Heteronucl_NOE_software_apps.Software_label -29 2 $nLinLS stop_ loop_ _Heteronucl_NOE_experiment.Heteronucl_NOE_list_ID _Heteronucl_NOE_experiment.Sample_ID _Heteronucl_NOE_experiment.Sample_label _Heteronucl_NOE_experiment.Experiment_ID _Heteronucl_NOE_experiment.Experiment_label 2 1 $sample1 . ? stop_ loop_ _Heteronucl_NOE.ID _Heteronucl_NOE.Sf_ID _Heteronucl_NOE.Heteronucl_NOE_list_ID _Heteronucl_NOE.Seq_ID_1 _Heteronucl_NOE.Seq_ID_2 _Heteronucl_NOE.Comp_ID_1 _Heteronucl_NOE.Comp_ID_2 _Heteronucl_NOE.Heteronucl_NOE_val _Heteronucl_NOE.Heteronucl_NOE_val_err _Heteronucl_NOE.Entity_assembly_ID_1 _Heteronucl_NOE.Entity_ID_1 _Heteronucl_NOE.Entity_assembly_ID_2 _Heteronucl_NOE.Entity_ID_2 _Heteronucl_NOE.Atom_ID_1 _Heteronucl_NOE.Atom_ID_2 1 -29 2 79 79 GLY GLY -0.3184 0.03 1 1 1 1 N H stop_ save_ save_entry_information _Entry.Sf_category entry_information _Entry.Sf_framecode entry_information _Entry.ID 5076 _Entry.Title ; Resonance Assignment of the unfolded states of Cold Shock Domain of the human YB-1 protein ; _Entry.Version_type new _Entry.Submission_date 2001-07-10 _Entry.Accession_date 2001-07-10 _Entry.Origination author _Entry.NMR_STAR_version "3.0 draft" _Entry.Experimental_method NMR _Entry.Details ; The data reported here represent the unfolded states of the single stranded DNA binding cold shock domain fragment (methionine plus residues 52-129) of the human Y box protein YB1 as present in solution. No denaturants are added to the samples. ; loop_ _Entry_author.Entry_ID _Entry_author.Ordinal _Entry_author.Family_name _Entry_author.Given_name _Entry_author.Middle_initials _Entry_author.Family_title 5076 1 Kloks Cathelijne 'P.A.M.' . 5076 2 Hoffmann Astrid . . 5076 3 Omichinski James G. . 5076 4 Vuister Geerten W. . 5076 5 Hilbers Cornelis W. . 5076 6 Grzesiek Stephan . . stop_ loop_ _Data_set.Entry_ID _Data_set.Type _Data_set.Count 5076 assigned_chemical_shifts 3 5076 coupling_constants 3 5076 T1_relaxation 6 5076 T1rho_relaxation 6 5076 heteronuclear_NOE 2 stop_ loop_ _Datum.Entry_ID _Datum.Type _Datum.Count 5076 '1H chemical shifts' 107 5076 '15N chemical shifts' 42 5076 '13C chemical shifts' 60 5076 'coupling constants' 17 5076 'T1 relaxation' 63 5076 'T1rho relaxation' 63 5076 'heteronuclear NOE' 28 stop_ loop_ _Release.Entry_ID _Release.Date _Release.Type _Release.Author _Release.Detail 5076 2002-11-19 update author "new data in chemical shift tables" stop_ loop_ _Related_entries.Entry_ID _Related_entries.BMRB_accession_code _Related_entries.Relationship 5076 4147 "data for folded protein" stop_ save_ save_entry_citation _Citation.Sf_category citations _Citation.Sf_framecode entry_citation _Citation.Entry_ID 5076 _Citation.ID 1 _Citation.Class entry_citation _Citation.MEDLINE_UI_code 99052121 _Citation.PubMed_ID 9835056 _Citation.Title ; Resonance Assignment and Secondary Structure of the Cold Shock Domain of the Human YB-1 Protein ; _Citation.Status published _Citation.Type journal _Citation.Journal_abbreviation 'J. Biomol. NMR' _Citation.Journal_volume 12 _Citation.Journal_issue 3 _Citation.Page_first 463 _Citation.Page_last 464 _Citation.Year 1998 loop_ _Citation_author.Citation_ID _Citation_author.Ordinal _Citation_author.Family_name _Citation_author.Given_name _Citation_author.Middle_initials _Citation_author.Family_title 1 1 Kloks Cathelijne 'P.A.M.' . 1 2 Hoffmann Astrid . . 1 3 Omichinski James G. . 1 4 Vuister Geerten W. . 1 5 Hilbers Cornelis W. . 1 6 Grzesiek Stephan . . stop_ loop_ _Citation_keyword.Citation_ID _Citation_keyword.Keyword 1 'cold shock domain' 1 'NMR assignments' 1 'OB fold' 1 'Y-box protein' 1 'unfolded' stop_ save_ # In the following saveframe, these tags were # missing from the order mapping rule in s2nmr, # and thus might not appear in order: # (SPECIAL: This name was duplicated, and only one # instance was preserved: _Entity_assembly.Assembly_ID ) save_system_YB1-CSD _Assembly.Sf_category assembly _Assembly.Sf_framecode system_YB1-CSD _Assembly.Entry_ID 5076 _Assembly.ID 1 _Assembly.Name 'Cold Shock domain' _Assembly.Paramagnetic no _Assembly.Thiol_state "not present" loop_ _Assembly_common_name.Assembly_ID _Assembly_common_name.Name 1 'Cold Shock domain' stop_ loop_ _Assembly_type.Assembly_ID _Assembly_type.Type 1 monomer stop_ loop_ _Entity_assembly.Assembly_ID _Entity_assembly.ID _Entity_assembly.Entity_ID _Entity_assembly.Entity_assembly_name _Entity_assembly.Entity_label _Entity_assembly.Magnetic_equivalence_group_code _Entity_assembly.Physical_state 1 1 1 'Cold Shock domain' $entity_YB1-CSD ? unfolded stop_ loop_ _Assembly_db_link.Assembly_ID _Assembly_db_link.Database_code _Assembly_db_link.Accession_code _Assembly_db_link.Entry_mol_name _Assembly_db_link.Entry_relation_type _Assembly_db_link.Entry_details 1 SWISS-PROT P16991 ? ? . 1 GenBank G340419 ? ? . stop_ save_ # In the following saveframe, these tags were # missing from the order mapping rule in s2nmr, # and thus might not appear in order: # _Entity.Polymer_common_type # _Entity_comp_index.Author_code save_entity_YB1-CSD _Entity.Sf_category entity _Entity.Sf_framecode YB1-CSD _Entity.Entry_ID 5076 _Entity.ID 1 _Entity.Name 'Cold Shock domain' _Entity.Type polymer _Entity.Polymer_type "polypeptide(L)" _Entity.Polymer_seq_one_letter_code ; MKKVIATKVLGTVKWFNVRN GYGFINRNDTKEDVFVHQTA IKKNNPRKYLRSVGDGETVE FDVVEGEKGAEAANVTGPG ; _Entity.Number_of_monomers 79 _Entity.DB_query_date 2003-07-24 _Entity.DB_query_revised_last_date 2003-06-26 loop_ _Entity_common_name.Entity_ID _Entity_common_name.Name 1 'Cold Shock domain' 1 YB1-CSD stop_ loop_ _Entity_poly_seq.Entity_ID _Entity_poly_seq.Num _Entity_poly_seq.Mon_ID 1 1 MET 1 2 LYS 1 3 LYS 1 4 VAL 1 5 ILE 1 6 ALA 1 7 THR 1 8 LYS 1 9 VAL 1 10 LEU 1 11 GLY 1 12 THR 1 13 VAL 1 14 LYS 1 15 TRP 1 16 PHE 1 17 ASN 1 18 VAL 1 19 ARG 1 20 ASN 1 21 GLY 1 22 TYR 1 23 GLY 1 24 PHE 1 25 ILE 1 26 ASN 1 27 ARG 1 28 ASN 1 29 ASP 1 30 THR 1 31 LYS 1 32 GLU 1 33 ASP 1 34 VAL 1 35 PHE 1 36 VAL 1 37 HIS 1 38 GLN 1 39 THR 1 40 ALA 1 41 ILE 1 42 LYS 1 43 LYS 1 44 ASN 1 45 ASN 1 46 PRO 1 47 ARG 1 48 LYS 1 49 TYR 1 50 LEU 1 51 ARG 1 52 SER 1 53 VAL 1 54 GLY 1 55 ASP 1 56 GLY 1 57 GLU 1 58 THR 1 59 VAL 1 60 GLU 1 61 PHE 1 62 ASP 1 63 VAL 1 64 VAL 1 65 GLU 1 66 GLY 1 67 GLU 1 68 LYS 1 69 GLY 1 70 ALA 1 71 GLU 1 72 ALA 1 73 ALA 1 74 ASN 1 75 VAL 1 76 THR 1 77 GLY 1 78 PRO 1 79 GLY stop_ loop_ _Entity_comp_index.Entity_ID _Entity_comp_index.ID _Entity_comp_index.Comp_ID _Entity_comp_index.Author_code 1 1 MET ? 1 2 LYS ? 1 3 LYS ? 1 4 VAL ? 1 5 ILE ? 1 6 ALA ? 1 7 THR ? 1 8 LYS ? 1 9 VAL ? 1 10 LEU ? 1 11 GLY ? 1 12 THR ? 1 13 VAL ? 1 14 LYS ? 1 15 TRP ? 1 16 PHE ? 1 17 ASN ? 1 18 VAL ? 1 19 ARG ? 1 20 ASN ? 1 21 GLY ? 1 22 TYR ? 1 23 GLY ? 1 24 PHE ? 1 25 ILE ? 1 26 ASN ? 1 27 ARG ? 1 28 ASN ? 1 29 ASP ? 1 30 THR ? 1 31 LYS ? 1 32 GLU ? 1 33 ASP ? 1 34 VAL ? 1 35 PHE ? 1 36 VAL ? 1 37 HIS ? 1 38 GLN ? 1 39 THR ? 1 40 ALA ? 1 41 ILE ? 1 42 LYS ? 1 43 LYS ? 1 44 ASN ? 1 45 ASN ? 1 46 PRO ? 1 47 ARG ? 1 48 LYS ? 1 49 TYR ? 1 50 LEU ? 1 51 ARG ? 1 52 SER ? 1 53 VAL ? 1 54 GLY ? 1 55 ASP ? 1 56 GLY ? 1 57 GLU ? 1 58 THR ? 1 59 VAL ? 1 60 GLU ? 1 61 PHE ? 1 62 ASP ? 1 63 VAL ? 1 64 VAL ? 1 65 GLU ? 1 66 GLY ? 1 67 GLU ? 1 68 LYS ? 1 69 GLY ? 1 70 ALA ? 1 71 GLU ? 1 72 ALA ? 1 73 ALA ? 1 74 ASN ? 1 75 VAL ? 1 76 THR ? 1 77 GLY ? 1 78 PRO ? 1 79 GLY ? stop_ loop_ _Entity_db_link.Entity_ID _Entity_db_link.Database_code _Entity_db_link.Accession_code _Entity_db_link.Entry_mol_name _Entity_db_link.Fragment_ID _Entity_db_link.Seq_homology_type _Entity_db_link.Seq_query_to_submitted_percent _Entity_db_link.Seq_subject_length _Entity_db_link.Seq_identity _Entity_db_link.Seq_positive _Entity_db_link.Seq_homology_expectation_val _Entity_db_link.Seq_difference_details _Entity_db_link.Seq_alignment_details 1 PDB 1H95 "A Chain A, Solution Structure Of TheSingle-Stranded Dna-Binding Cold Shock Domain (Csd) OfHuman Y-Box Protein 1 (Yb1) Determined By Nmr (10Lowest Energy Structures)" ? ? 100.00 79 100 100 1e-39 ? ? 1 DBJ BAC45236.1 "Y-box binding protein [Oryzias latipes]" ? ? 25.82 306 100 100 1e-39 ? ? 1 DBJ BAA03376.1 "dbpA murine homologue [Mus musculus]" ? ? 25.65 308 99 100 1e-38 ? ? 1 DBJ BAA19849.1 "Y box protein 1 [Carassius auratus]" ? ? 25.40 311 100 100 1e-39 ? ? 1 DBJ BAA02569.1 "DNA binding protein B [Rattus sp.]" ? ? 24.53 322 100 100 1e-39 ? ? 1 DBJ BAA05380.1 "similar to dbpB/YB-1 of mouse [Gallusgallus]" ? ? 24.23 326 100 100 1e-39 ? ? 1 EMBL CAC39432.1 "cold-shock domain protein [Takifugurubripes]" ? ? 30.04 263 100 100 1e-39 ? ? 1 EMBL CAA42778.1 "YB3 [Xenopus laevis]" ? ? 25.90 305 100 100 1e-39 ? ? 1 EMBL CAA40847.1 "Y box-binbing protein [Mus musculus]" ? ? 24.61 321 100 100 1e-39 ? ? 1 EMBL CAA51261.1 "cold shock domain protein A [Homosapiens]" ? ? 21.24 372 99 100 1e-38 ? ? 1 EMBL CAA64631.1 "DNA-binding protein [Homo sapiens]" ? ? 21.24 372 99 100 1e-38 ? ? 1 GenBank AAB94768.1 "Y box transcription factor [Mus musculus]" ? ? 26.42 299 100 100 1e-39 ? ? 1 GenBank AAC62774.1 "zfY1 [Danio rerio]" ? ? 25.48 310 100 100 1e-39 ? ? 1 GenBank AAA59949.1 "DNA-binding protein" ? ? 24.69 320 100 100 1e-39 ? ? 1 GenBank AAA02573.1 "YB-1 protein" ? ? 24.61 321 100 100 1e-39 ? ? 1 GenBank AAA41108.1 "enhancer factor-1-alpha" ? ? 24.53 322 100 100 1e-39 ? ? 1 PIR S22313 "B box-binding protein - African clawed frog" ? ? 25.90 305 100 100 1e-39 ? ? 1 PIR A48136 "Y box-binding protein 1 - chicken" ? ? 24.61 321 100 100 1e-39 ? ? 1 PIR A23677 "Y box-binding protein 1 - rat" ? ? 24.53 322 100 100 1e-39 ? ? 1 PIR I39382 "Y box-binding protein 1 - human" ? ? 24.38 324 100 100 1e-39 ? ? 1 PIR JQ2292 "Y box-binding protein 1 - bovine" ? ? 24.38 324 100 100 1e-39 ? ? 1 PRF 2210343A "nicotinic acetylcholine receptor repressor" ? ? 21.88 361 99 100 1e-38 ? ? 1 REF NP_571695.1 "nuclease sensitive element bindingprotein 1; Y-box binding protein 1 [Danio rerio]" ? ? 25.48 310 100 100 1e-39 ? ? 1 REF NP_035862.1 "nuclease sensitive element bindingprotein 1; Y box protein 1; DNA binding protein B; Y-boxbinding protein 1 [Mus musculus]" ? ? 24.61 321 100 100 1e-39 ? ? 1 REF NP_004550.1 "nuclease sensitive element bindingprotein 1; major histocompatibility complex, class II, Ybox-binding protein I; DNA-binding protein B [Homosapiens]" ? ? 24.53 322 100 100 1e-39 ? ? 1 REF NP_113751.2 "nuclease sensitive element bindingprotein 1; Y box protein 1 [Rattus norvegicus]" ? ? 24.53 322 100 100 1e-39 ? ? 1 REF NP_777240.1 "nuclease sensitive element bindingprotein 1 [Bos taurus]" ? ? 24.38 324 100 100 1e-39 ? ? 1 SWISS-PROT P21573 "YB1_XENLA Nuclease sensitive element bindingprotein 1 (Y box binding protein-1) (Y-box transcriptionfactor)" ? ? 26.07 303 100 100 7e-39 ? ? 1 SWISS-PROT Q00436 "YB3_XENLA B BOX BINDING PROTEIN (YB3 PROTEIN)" ? ? 25.90 305 100 100 1e-39 ? ? 1 SWISS-PROT Q06066 "YB1_CHICK Nuclease sensitive element bindingprotein 1 (Y box binding protein-1) (Y-box transcriptionfactor)" ? ? 24.61 321 100 100 1e-39 ? ? 1 SWISS-PROT P27817 "YB1_MOUSE Nuclease sensitive element bindingprotein 1 (Y box binding protein-1) (Y-box transcriptionfactor) (YB-1) (CCAAT-binding transcription factor Isubunit A) (CBF-A) (Enhancer factor I subunit A) (EFI-A)(DNA-binding protein B) (DBPB)" ? ? 24.53 322 100 100 1e-39 ? ? 1 SWISS-PROT P16991 "YB1_HUMAN Nuclease sensitive element bindingprotein 1 (Y box binding protein-1) (Y-box transcriptionfactor) (YB-1) (CCAAT-binding transcription factor Isubunit A) (CBF-A) (Enhancer factor I subunit A) (EFI-A)(DNA-binding protein B) (DBPB)" ? ? 24.38 324 100 100 1e-39 ? ? stop_ _Entity.Polymer_common_type protein save_ save_natural_source _Entity_natural_src_list.Sf_category natural_source _Entity_natural_src_list.Sf_framecode natural_source _Entity_natural_src_list.Entry_ID 5076 _Entity_natural_src_list.ID 1 loop_ _Entity_natural_src.Entity_natural_src_list_ID _Entity_natural_src.Entity_ID _Entity_natural_src.Entity_label _Entity_natural_src.Organism_name_common _Entity_natural_src.Organism_acronym _Entity_natural_src.ICTVdb_decimal_code _Entity_natural_src.NCBI_taxonomy_ID _Entity_natural_src.Superkingdom _Entity_natural_src.Kingdom _Entity_natural_src.Genus _Entity_natural_src.Species _Entity_natural_src.Strain _Entity_natural_src.Variant _Entity_natural_src.Subvariant _Entity_natural_src.Organ _Entity_natural_src.Tissue _Entity_natural_src.Cell_line _Entity_natural_src.Cell_type _Entity_natural_src.ATCC_number _Entity_natural_src.Organelle _Entity_natural_src.Fraction _Entity_natural_src.Secretion _Entity_natural_src.Plasmid _Entity_natural_src.Gene_mnemonic _Entity_natural_src.Details 1 1 $entity_YB1-CSD human ? ? 9606 Eukaryota Metazoa Homo sapiens ? ? ? ? ? ? ? ? ? ? ? ? ? ? stop_ save_ save_experimental_source _Entity_experimental_src_list.Sf_category experimental_source _Entity_experimental_src_list.Sf_framecode experimental_source _Entity_experimental_src_list.Entry_ID 5076 _Entity_experimental_src_list.ID 1 loop_ _Entity_experimental_src.Entity_experimental_src_list_ID _Entity_experimental_src.Entity_ID _Entity_experimental_src.Entity_label _Entity_experimental_src.Production_method _Entity_experimental_src.Host_organism_name_common _Entity_experimental_src.Genus _Entity_experimental_src.Species _Entity_experimental_src.Strain _Entity_experimental_src.Variant _Entity_experimental_src.Organ _Entity_experimental_src.Tissue _Entity_experimental_src.Cell_line _Entity_experimental_src.Cell_type _Entity_experimental_src.ATCC_number _Entity_experimental_src.Vector_type _Entity_experimental_src.Vector_name _Entity_experimental_src.Vendor_name _Entity_experimental_src.Gene_source _Entity_experimental_src.Details 1 1 $entity_YB1-CSD 'recombinant technology' 'E. coli' Escherichia coli BL21(DE3) ? ? ? ? ? ? plasmid pET11a ? ? ? stop_ save_ save_sample1 _Sample.Sf_category sample _Sample.Sf_framecode sample1 _Sample.Entry_ID 5076 _Sample.ID 1 _Sample.Type solution loop_ _Sample_component.Sample_ID _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Concentration_val _Sample_component.Concentration_val_units _Sample_component.Concentration_min_val _Sample_component.Concentration_max_val _Sample_component.Isotopic_labeling 1 1 . . . 1 $entity_YB1-CSD 1.5 mM ? ? [U-15N] 1 2 H2O . . . . 90 % ? ? . 1 3 D2O . . . . 10 % ? ? . stop_ save_ save_sample2 _Sample.Sf_category sample _Sample.Sf_framecode sample2 _Sample.Entry_ID 5076 _Sample.ID 2 _Sample.Type solution loop_ _Sample_component.Sample_ID _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Concentration_val _Sample_component.Concentration_val_units _Sample_component.Concentration_min_val _Sample_component.Concentration_max_val _Sample_component.Isotopic_labeling 2 1 . . . 1 $entity_YB1-CSD 1.5 mM ? ? "[U-15N; U-13C]" 2 2 H2O . . . . 90 % ? ? . 2 3 D2O . . . . 10 % ? ? . stop_ save_ save_sample3 _Sample.Sf_category sample _Sample.Sf_framecode sample3 _Sample.Entry_ID 5076 _Sample.ID 3 _Sample.Type solution loop_ _Sample_component.Sample_ID _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Concentration_val _Sample_component.Concentration_val_units _Sample_component.Concentration_min_val _Sample_component.Concentration_max_val _Sample_component.Isotopic_labeling 3 1 . . . 1 $entity_YB1-CSD 1.5 mM ? ? "[U-15N; U-13C]" 3 2 D2O . . . . 100 % ? ? . stop_ save_ # In the following saveframe, these tags were # missing from the order mapping rule in s2nmr, # and thus might not appear in order: # _Chem_shift_ref.Atom_isotope_number # _Chem_shift_ref.Correction_val_Citation_ID # _Chem_shift_ref.Correction_val_cit_label save_chemical_shift_reference _Chem_shift_reference.Sf_category chem_shift_reference _Chem_shift_reference.Sf_framecode chemical_shift_reference _Chem_shift_reference.Entry_ID 5076 _Chem_shift_reference.ID 1 loop_ _Chem_shift_ref.Chem_shift_reference_ID _Chem_shift_ref.Mol_common_name _Chem_shift_ref.Atom_type _Chem_shift_ref.Atom_group _Chem_shift_ref.Chem_shift_units _Chem_shift_ref.Chem_shift_val _Chem_shift_ref.Ref_method _Chem_shift_ref.Ref_type _Chem_shift_ref.External_ref_sample_geometry _Chem_shift_ref.External_ref_loc _Chem_shift_ref.External_ref_axis _Chem_shift_ref.Indirect_shift_ratio _Chem_shift_ref.Indirect_shift_ratio_Cit_ID _Chem_shift_ref.Indirect_shift_ratio_Cit_label _Chem_shift_ref.Ref_correction_type _Chem_shift_ref.Correction_val _Chem_shift_ref.Correction_val_Cit_label _Chem_shift_ref.Atom_isotope_number _Chem_shift_ref.Correction_val_Citation_ID _Chem_shift_ref.Correction_val_cit_label 1 TSP H 'methyl protons' ppm 0.00 . direct ? ? ? . . ? ? ? ? 1 . ? 1 'liquid NH3' N nitrogen ppm 0.00 . indirect ? ? ? . . ? ? ? ? 15 . ? stop_ save_ save_assigned_chemical_shifts_one _Assigned_chem_shift_list.Sf_category assigned_chemical_shifts _Assigned_chem_shift_list.Sf_framecode assigned_chemical_shifts_one _Assigned_chem_shift_list.Entry_ID 5076 _Assigned_chem_shift_list.ID 1 _Assigned_chem_shift_list.Details ; Major unfolded state of cold shock domain of human YB-1 protein. ; _Assigned_chem_shift_list.Sample_conditions_label $sample_conditions_one _Assigned_chem_shift_list.Chem_shift_reference_label $chemical_shift_reference loop_ _Chem_shift_experiment.Assigned_chem_shift_list_ID _Chem_shift_experiment.Experiment_ID _Chem_shift_experiment.Experiment_label _Chem_shift_experiment.Sample_ID _Chem_shift_experiment.Sample_label 1 . ? 1 $sample1 1 . ? 2 $sample2 1 . ? 3 $sample3 stop_ loop_ _Atom_chem_shift.Assigned_chem_shift_list_ID _Atom_chem_shift.ID _Atom_chem_shift.Entity_assembly_ID _Atom_chem_shift.Entity_ID _Atom_chem_shift.Comp_index_ID _Atom_chem_shift.Seq_ID _Atom_chem_shift.Comp_ID _Atom_chem_shift.Atom_ID _Atom_chem_shift.Atom_type _Atom_chem_shift.Chem_shift_val _Atom_chem_shift.Chem_shift_val_err _Atom_chem_shift.Chem_shift_ambiguity_code 1 1 1 1 3 3 LYS H H 8.560 . 1 1 2 1 1 3 3 LYS N N 123.984 . 1 1 3 1 1 4 4 VAL HA H 4.18 . 1 1 4 1 1 4 4 VAL CA C 62.22 . 1 1 5 1 1 5 5 ILE H H 8.306 . 1 1 6 1 1 5 5 ILE CA C 60.70 . 1 1 7 1 1 5 5 ILE N N 125.984 . 1 1 8 1 1 6 6 ALA H H 8.407 . 1 1 9 1 1 6 6 ALA HB H 1.41 . 1 1 10 1 1 6 6 ALA N N 129.010 . 1 1 11 1 1 9 9 VAL H H 8.145 . 1 1 12 1 1 9 9 VAL N N 123.425 . 1 1 13 1 1 10 10 LEU H H 8.333 . 1 1 14 1 1 10 10 LEU HD2 H 0.71 . 2 1 15 1 1 10 10 LEU N N 122.150 . 1 1 16 1 1 11 11 GLY H H 8.207 . 1 1 17 1 1 11 11 GLY N N 108.537 . 1 1 18 1 1 12 12 THR H H 8.000 . 1 1 19 1 1 12 12 THR N N 113.90 . 1 1 20 1 1 15 15 TRP HD1 H 7.13 . 1 1 21 1 1 15 15 TRP HE1 H 10.094 . 1 1 22 1 1 15 15 TRP NE1 N 129.384 . 1 1 23 1 1 16 16 PHE H H 7.91 . 1 1 24 1 1 19 19 ARG H H 8.056 . 1 1 25 1 1 19 19 ARG N N 120.022 . 1 1 26 1 1 25 25 ILE H H 8.05 . 1 1 27 1 1 25 25 ILE HD1 H 0.73 . 1 1 28 1 1 26 26 ASN HD21 H 7.57 . 2 1 29 1 1 26 26 ASN HD22 H 6.91 . 2 1 30 1 1 27 27 ARG H H 8.35 . 1 1 31 1 1 28 28 ASN H H 8.49 . 1 1 32 1 1 29 29 ASP H H 8.30 . 1 1 33 1 1 29 29 ASP HA H 4.66 . 1 1 34 1 1 29 29 ASP HB2 H 2.74 . 2 1 35 1 1 29 29 ASP HB3 H 2.83 . 2 1 36 1 1 29 29 ASP CA C 54.43 . 1 1 37 1 1 29 29 ASP CB C 41.21 . 1 1 38 1 1 30 30 THR H H 8.05 . 1 1 39 1 1 30 30 THR CA C 61.93 . 1 1 40 1 1 30 30 THR CB C 69.94 . 1 1 41 1 1 30 30 THR N N 114.023 . 1 1 42 1 1 31 31 LYS H H 8.302 . 1 1 43 1 1 31 31 LYS N N 123.152 . 1 1 44 1 1 32 32 GLU H H 8.36 . 1 1 45 1 1 32 32 GLU N N 121.25 . 1 1 46 1 1 33 33 ASP H H 8.36 . 1 1 47 1 1 33 33 ASP HA H 4.58 . 1 1 48 1 1 33 33 ASP HB2 H 2.66 . 2 1 49 1 1 33 33 ASP CA C 54.43 . 1 1 50 1 1 33 33 ASP CB C 41.21 . 1 1 51 1 1 34 34 VAL H H 7.900 . 1 1 52 1 1 34 34 VAL N N 119.185 . 1 1 53 1 1 35 35 PHE H H 8.21 . 1 1 54 1 1 35 35 PHE HA H 4.74 . 1 1 55 1 1 35 35 PHE HB2 H 2.82 . 2 1 56 1 1 35 35 PHE HB3 H 3.07 . 2 1 57 1 1 35 35 PHE CA C 57.86 . 1 1 58 1 1 35 35 PHE CB C 39.50 . 1 1 59 1 1 35 35 PHE N N 122.48 . 1 1 60 1 1 36 36 VAL H H 7.89 . 1 1 61 1 1 36 36 VAL HA H 3.99 . 1 1 62 1 1 36 36 VAL N N 121.32 . 1 1 63 1 1 40 40 ALA H H 8.156 . 1 1 64 1 1 40 40 ALA HA H 3.81 . 1 1 65 1 1 40 40 ALA CA C 56.64 . 1 1 66 1 1 40 40 ALA CB C 19.32 . 1 1 67 1 1 40 40 ALA N N 114.747 . 1 1 68 1 1 41 41 ILE H H 7.962 . 1 1 69 1 1 41 41 ILE HD1 H 0.77 . 1 1 70 1 1 41 41 ILE CA C 61.76 . 1 1 71 1 1 41 41 ILE CB C 37.40 . 1 1 72 1 1 41 41 ILE N N 119.673 . 1 1 73 1 1 42 42 LYS H H 8.801 . 1 1 74 1 1 42 42 LYS N N 129.775 . 1 1 75 1 1 50 50 LEU H H 8.11 . 1 1 76 1 1 54 54 GLY HA3 H 3.73 . 2 1 77 1 1 54 54 GLY HA2 H 4.26 . 2 1 78 1 1 54 54 GLY CA C 44.40 . 1 1 79 1 1 55 55 ASP H H 8.361 . 1 1 80 1 1 55 55 ASP HA H 4.03 . 1 1 81 1 1 55 55 ASP CA C 54.64 . 1 1 82 1 1 55 55 ASP CB C 41.20 . 1 1 83 1 1 55 55 ASP N N 120.104 . 1 1 84 1 1 56 56 GLY H H 8.357 . 1 1 85 1 1 56 56 GLY HA3 H 3.90 . 2 1 86 1 1 56 56 GLY HA2 H 4.14 . 2 1 87 1 1 56 56 GLY CA C 45.58 . 1 1 88 1 1 56 56 GLY N N 109.079 . 1 1 89 1 1 57 57 GLU H H 8.187 . 1 1 90 1 1 57 57 GLU CA C 56.47 . 1 1 91 1 1 57 57 GLU CB C 30.73 . 1 1 92 1 1 57 57 GLU CG C 36.43 . 1 1 93 1 1 57 57 GLU N N 120.313 . 1 1 94 1 1 58 58 THR H H 8.330 . 1 1 95 1 1 58 58 THR HG2 H 1.24 . 1 1 96 1 1 58 58 THR N N 115.682 . 1 1 97 1 1 59 59 VAL H H 8.22 . 1 1 98 1 1 60 60 GLU H H 8.331 . 1 1 99 1 1 60 60 GLU HA H 4.38 . 1 1 100 1 1 60 60 GLU CA C 45.46 . 1 1 101 1 1 60 60 GLU N N 124.084 . 1 1 102 1 1 61 61 PHE H H 8.229 . 1 1 103 1 1 61 61 PHE N N 120.686 . 1 1 104 1 1 62 62 ASP HA H 4.21 . 1 1 105 1 1 62 62 ASP CA C 54.10 . 1 1 106 1 1 63 63 VAL H H 8.082 . 1 1 107 1 1 63 63 VAL CA C 62.29 . 1 1 108 1 1 63 63 VAL N N 120.334 . 1 1 109 1 1 64 64 VAL H H 8.223 . 1 1 110 1 1 64 64 VAL HA H 4.12 . 1 1 111 1 1 64 64 VAL HB H 2.03 . 1 1 112 1 1 64 64 VAL HG2 H 0.89 . 2 1 113 1 1 64 64 VAL CA C 62.23 . 1 1 114 1 1 64 64 VAL N N 124.095 . 1 1 115 1 1 65 65 GLU H H 8.462 . 1 1 116 1 1 65 65 GLU HA H 4.26 . 1 1 117 1 1 65 65 GLU HB2 H 2.08 . 2 1 118 1 1 65 65 GLU HG2 H 2.23 . 2 1 119 1 1 65 65 GLU CA C 56.87 . 1 1 120 1 1 65 65 GLU CB C 30.66 . 1 1 121 1 1 65 65 GLU N N 124.817 . 1 1 122 1 1 66 66 GLY H H 8.357 . 1 1 123 1 1 66 66 GLY CA C 45.56 . 1 1 124 1 1 66 66 GLY N N 109.922 . 1 1 125 1 1 67 67 GLU H H 8.27 . 1 1 126 1 1 67 67 GLU CA C 56.67 . 1 1 127 1 1 67 67 GLU CB C 30.44 . 1 1 128 1 1 67 67 GLU CG C 36.43 . 1 1 129 1 1 68 68 LYS HA H 4.34 . 1 1 130 1 1 68 68 LYS HB2 H 1.87 . 2 1 131 1 1 68 68 LYS CA C 56.70 . 1 1 132 1 1 68 68 LYS CB C 33.00 . 1 1 133 1 1 68 68 LYS CG C 25.28 . 1 1 134 1 1 69 69 GLY H H 8.436 . 1 1 135 1 1 69 69 GLY HA3 H 3.90 . 1 1 136 1 1 69 69 GLY HA2 H 3.90 . 1 1 137 1 1 69 69 GLY CA C 45.39 . 1 1 138 1 1 69 69 GLY N N 110.096 . 1 1 139 1 1 70 70 ALA H H 8.167 . 1 1 140 1 1 70 70 ALA HA H 4.30 . 1 1 141 1 1 70 70 ALA CA C 52.74 . 1 1 142 1 1 70 70 ALA CB C 19.36 . 1 1 143 1 1 70 70 ALA N N 123.755 . 1 1 144 1 1 71 71 GLU H H 8.46 . 1 1 145 1 1 71 71 GLU HA H 4.24 . 1 1 146 1 1 71 71 GLU HB2 H 2.10 . 2 1 147 1 1 71 71 GLU HB3 H 1.98 . 2 1 148 1 1 71 71 GLU CA C 56.87 . 1 1 149 1 1 71 71 GLU CB C 30.21 . 1 1 150 1 1 71 71 GLU CG C 36.39 . 1 1 151 1 1 71 71 GLU N N 119.68 . 1 1 152 1 1 72 72 ALA H H 8.197 . 1 1 153 1 1 72 72 ALA HA H 4.29 . 1 1 154 1 1 72 72 ALA HB H 1.43 . 1 1 155 1 1 72 72 ALA CA C 52.56 . 1 1 156 1 1 72 72 ALA CB C 19.34 . 1 1 157 1 1 72 72 ALA N N 124.674 . 1 1 158 1 1 73 73 ALA H H 8.148 . 1 1 159 1 1 73 73 ALA CA C 52.59 . 1 1 160 1 1 73 73 ALA CB C 19.39 . 1 1 161 1 1 73 73 ALA N N 122.547 . 1 1 162 1 1 74 74 ASN H H 9.291 . 1 1 163 1 1 74 74 ASN N N 118.058 . 1 1 164 1 1 75 75 VAL H H 9.55 . 1 1 165 1 1 75 75 VAL HA H 4.26 . 1 1 166 1 1 75 75 VAL CA C 65.52 . 1 1 167 1 1 75 75 VAL CB C 32.76 . 1 1 168 1 1 75 75 VAL N N 117.135 . 1 1 169 1 1 76 76 THR H H 8.22 . 1 1 170 1 1 76 76 THR HA H 4.41 . 1 1 171 1 1 76 76 THR CA C 61.80 . 1 1 172 1 1 76 76 THR CB C 70.03 . 1 1 173 1 1 77 77 GLY CA C 44.71 . 1 1 174 1 1 78 78 PRO HA H 4.46 . 1 1 175 1 1 78 78 PRO HB2 H 2.07 . 2 1 176 1 1 78 78 PRO HB3 H 2.27 . 2 1 177 1 1 78 78 PRO HD2 H 3.66 . 2 1 178 1 1 78 78 PRO HD3 H 3.58 . 2 1 179 1 1 78 78 PRO CA C 63.45 . 1 1 180 1 1 78 78 PRO CB C 32.35 . 1 1 181 1 1 78 78 PRO CG C 27.33 . 1 1 182 1 1 79 79 GLY H H 8.015 . 1 1 183 1 1 79 79 GLY HA2 H 3.75 . 2 1 184 1 1 79 79 GLY CA C 46.36 . 1 1 185 1 1 79 79 GLY N N 115.347 . 1 stop_ save_ save_assigned_chemical_shifts_two _Assigned_chem_shift_list.Sf_category assigned_chemical_shifts _Assigned_chem_shift_list.Sf_framecode assigned_chemical_shifts_two _Assigned_chem_shift_list.Entry_ID 5076 _Assigned_chem_shift_list.ID 2 _Assigned_chem_shift_list.Details ; Minor unfolded state of cold shock domain of human YB-1 protein. ; _Assigned_chem_shift_list.Sample_conditions_label $sample_conditions_one _Assigned_chem_shift_list.Chem_shift_reference_label $chemical_shift_reference loop_ _Chem_shift_experiment.Assigned_chem_shift_list_ID _Chem_shift_experiment.Experiment_ID _Chem_shift_experiment.Experiment_label _Chem_shift_experiment.Sample_ID _Chem_shift_experiment.Sample_label 2 . ? 1 $sample1 2 . ? 2 $sample2 2 . ? 3 $sample3 stop_ loop_ _Atom_chem_shift.Assigned_chem_shift_list_ID _Atom_chem_shift.ID _Atom_chem_shift.Entity_assembly_ID _Atom_chem_shift.Entity_ID _Atom_chem_shift.Comp_index_ID _Atom_chem_shift.Seq_ID _Atom_chem_shift.Comp_ID _Atom_chem_shift.Atom_ID _Atom_chem_shift.Atom_type _Atom_chem_shift.Chem_shift_val _Atom_chem_shift.Chem_shift_val_err _Atom_chem_shift.Chem_shift_ambiguity_code 2 1 1 1 11 11 GLY H H 8.32 . 1 2 2 1 1 11 11 GLY N N 109.48 . 1 2 3 1 1 42 42 LYS H H 8.826 . 1 2 4 1 1 42 42 LYS HA H 4.28 . 1 2 5 1 1 42 42 LYS CB C 32.93 . 1 2 6 1 1 42 42 LYS N N 129.264 . 1 2 7 1 1 71 71 GLU H H 8.359 . 1 2 8 1 1 71 71 GLU N N 121.627 . 1 2 9 1 1 74 74 ASN H H 8.333 . 1 2 10 1 1 74 74 ASN N N 117.824 . 1 2 11 1 1 78 78 PRO HA H 4.64 . 1 2 12 1 1 78 78 PRO CA C 63.10 . 1 2 13 1 1 78 78 PRO CB C 34.56 . 1 2 14 1 1 79 79 GLY H H 8.240 . 1 2 15 1 1 79 79 GLY HA2 H 3.76 . 2 2 16 1 1 79 79 GLY CA C 46.41 . 1 2 17 1 1 79 79 GLY N N 116.715 . 1 stop_ save_ save_assigned_chemical_shifts_three _Assigned_chem_shift_list.Sf_category assigned_chemical_shifts _Assigned_chem_shift_list.Sf_framecode assigned_chemical_shifts_three _Assigned_chem_shift_list.Entry_ID 5076 _Assigned_chem_shift_list.ID 3 _Assigned_chem_shift_list.Details ; Additional minor unfolded state of cold shock domain of human YB-1 protein. ; _Assigned_chem_shift_list.Sample_conditions_label $sample_conditions_one _Assigned_chem_shift_list.Chem_shift_reference_label $chemical_shift_reference loop_ _Chem_shift_experiment.Assigned_chem_shift_list_ID _Chem_shift_experiment.Experiment_ID _Chem_shift_experiment.Experiment_label _Chem_shift_experiment.Sample_ID _Chem_shift_experiment.Sample_label 3 . ? 1 $sample1 3 . ? 2 $sample2 3 . ? 3 $sample3 stop_ loop_ _Atom_chem_shift.Assigned_chem_shift_list_ID _Atom_chem_shift.ID _Atom_chem_shift.Entity_assembly_ID _Atom_chem_shift.Entity_ID _Atom_chem_shift.Comp_index_ID _Atom_chem_shift.Seq_ID _Atom_chem_shift.Comp_ID _Atom_chem_shift.Atom_ID _Atom_chem_shift.Atom_type _Atom_chem_shift.Chem_shift_val _Atom_chem_shift.Chem_shift_val_err _Atom_chem_shift.Chem_shift_ambiguity_code 3 1 1 1 78 78 PRO HA H 4.77 . 1 3 2 1 1 78 78 PRO CA C 62.50 . 1 3 3 1 1 78 78 PRO CB C 34.90 . 1 3 4 1 1 79 79 GLY H H 8.410 . 1 3 5 1 1 79 79 GLY HA2 H 3.59 . 2 3 6 1 1 79 79 GLY CA C 46.41 . 1 3 7 1 1 79 79 GLY N N 116.806 . 1 stop_ save_ # In the following saveframe, these tags were # missing from the order mapping rule in s2nmr, # and thus might not appear in order: # _Coupling_constant.Coupling_constant_list_ID save_coupling_constants_3jhnha_major_unfolded_state _Coupling_constant_list.Sf_category coupling_constants _Coupling_constant_list.Sf_framecode coupling_constants_3jhnha_major_unfolded_state _Coupling_constant_list.Entry_ID 5076 _Coupling_constant_list.ID 1 _Coupling_constant_list.Details ; The theoretical correction factor is 5%. ; _Coupling_constant_list.Sample_conditions_ID 1 _Coupling_constant_list.Sample_conditions_label $sample_conditions_one loop_ _Coupling_constant_experiment.Coupling_constant_list_ID _Coupling_constant_experiment.Experiment_ID _Coupling_constant_experiment.Experiment_label _Coupling_constant_experiment.Sample_ID _Coupling_constant_experiment.Sample_label 1 . ? 1 $sample1 stop_ loop_ _Coupling_constant.ID _Coupling_constant.Code _Coupling_constant.Entity_assembly_ID_1 _Coupling_constant.Entity_ID_1 _Coupling_constant.Comp_index_ID_1 _Coupling_constant.Seq_ID_1 _Coupling_constant.Comp_ID_1 _Coupling_constant.Atom_ID_1 _Coupling_constant.Entity_assembly_ID_2 _Coupling_constant.Entity_ID_2 _Coupling_constant.Comp_index_ID_2 _Coupling_constant.Seq_ID_2 _Coupling_constant.Comp_ID_2 _Coupling_constant.Atom_ID_2 _Coupling_constant.Val _Coupling_constant.Min_val _Coupling_constant.Max_val _Coupling_constant.Val_err _Coupling_constant.Coupling_constant_list_ID 1 3JHNHA 1 1 19 19 ARG HA 1 1 19 19 ARG H 7.8 ? ? . 1 2 3JHNHA 1 1 34 34 VAL HA 1 1 34 34 VAL H 7.2 ? ? . 1 3 3JHNHA 1 1 41 41 ILE HA 1 1 41 41 ILE H 7.5 ? ? . 1 4 3JHNHA 1 1 42 42 LYS HA 1 1 42 42 LYS H 7.1 ? ? . 1 5 3JHNHA 1 1 57 57 GLU HA 1 1 57 57 GLU H 6.2 ? ? . 1 6 3JHNHA 1 1 60 60 GLU HA 1 1 60 60 GLU H 6.8 ? ? . 1 7 3JHNHA 1 1 61 61 PHE HA 1 1 61 61 PHE H 6.7 ? ? . 1 8 3JHNHA 1 1 63 63 VAL HA 1 1 63 63 VAL H 7.6 ? ? . 1 9 3JHNHA 1 1 65 65 GLU HA 1 1 65 65 GLU H 6.2 ? ? . 1 10 3JHNHA 1 1 66 66 GLY HA 1 1 66 66 GLY H 8.3 ? ? . 1 11 3JHNHA 1 1 69 69 GLY HA 1 1 69 69 GLY H 10.8 ? ? . 1 12 3JHNHA 1 1 71 71 GLU HA 1 1 71 71 GLU H 6.8 ? ? . 1 13 3JHNHA 1 1 72 72 ALA HA 1 1 72 72 ALA H 5.9 ? ? . 1 14 3JHNHA 1 1 79 79 GLY HA 1 1 79 79 GLY H 7.3 ? ? . 1 stop_ save_ save_coupling_constants_3jhnha_minor_unfolded_state _Coupling_constant_list.Sf_category coupling_constants _Coupling_constant_list.Sf_framecode coupling_constants_3jhnha_minor_unfolded_state _Coupling_constant_list.Entry_ID 5076 _Coupling_constant_list.ID 2 _Coupling_constant_list.Details ; The theoretical correction factor is 5%. ; _Coupling_constant_list.Sample_conditions_ID 1 _Coupling_constant_list.Sample_conditions_label $sample_conditions_one loop_ _Coupling_constant_experiment.Coupling_constant_list_ID _Coupling_constant_experiment.Experiment_ID _Coupling_constant_experiment.Experiment_label _Coupling_constant_experiment.Sample_ID _Coupling_constant_experiment.Sample_label 2 . ? 1 $sample1 stop_ loop_ _Coupling_constant.ID _Coupling_constant.Code _Coupling_constant.Entity_assembly_ID_1 _Coupling_constant.Entity_ID_1 _Coupling_constant.Comp_index_ID_1 _Coupling_constant.Seq_ID_1 _Coupling_constant.Comp_ID_1 _Coupling_constant.Atom_ID_1 _Coupling_constant.Entity_assembly_ID_2 _Coupling_constant.Entity_ID_2 _Coupling_constant.Comp_index_ID_2 _Coupling_constant.Seq_ID_2 _Coupling_constant.Comp_ID_2 _Coupling_constant.Atom_ID_2 _Coupling_constant.Val _Coupling_constant.Min_val _Coupling_constant.Max_val _Coupling_constant.Val_err _Coupling_constant.Coupling_constant_list_ID 1 3JHNHA 1 1 42 42 LYS HA 1 1 42 42 LYS H 9.8 ? ? . 2 2 3JHNHA 1 1 79 79 GLY HA 1 1 79 79 GLY H 7.3 ? ? . 2 stop_ save_ save_coupling_constants_3jhnha_extra_minor_unfolded_state _Coupling_constant_list.Sf_category coupling_constants _Coupling_constant_list.Sf_framecode coupling_constants_3jhnha_extra_minor_unfolded_state _Coupling_constant_list.Entry_ID 5076 _Coupling_constant_list.ID 3 _Coupling_constant_list.Details ; The theoretical correction factor is 5%. ; _Coupling_constant_list.Sample_conditions_ID 1 _Coupling_constant_list.Sample_conditions_label $sample_conditions_one loop_ _Coupling_constant_experiment.Coupling_constant_list_ID _Coupling_constant_experiment.Experiment_ID _Coupling_constant_experiment.Experiment_label _Coupling_constant_experiment.Sample_ID _Coupling_constant_experiment.Sample_label 3 . ? 1 $sample1 stop_ loop_ _Coupling_constant.ID _Coupling_constant.Code _Coupling_constant.Entity_assembly_ID_1 _Coupling_constant.Entity_ID_1 _Coupling_constant.Comp_index_ID_1 _Coupling_constant.Seq_ID_1 _Coupling_constant.Comp_ID_1 _Coupling_constant.Atom_ID_1 _Coupling_constant.Entity_assembly_ID_2 _Coupling_constant.Entity_ID_2 _Coupling_constant.Comp_index_ID_2 _Coupling_constant.Seq_ID_2 _Coupling_constant.Comp_ID_2 _Coupling_constant.Atom_ID_2 _Coupling_constant.Val _Coupling_constant.Min_val _Coupling_constant.Max_val _Coupling_constant.Val_err _Coupling_constant.Coupling_constant_list_ID 1 3JHNHA 1 1 79 79 GLY HA 1 1 79 79 GLY H 7.4 ? ? . 3 stop_ save_ save_nLinLS _Software.Sf_category software _Software.Sf_framecode nLinLS _Software.Entry_ID 5076 _Software.ID 1 _Software.Name nmrPipe _Software.Version ? _Software.Details ; The nLinLS program is part of the nmrPipe package and is used to fit the exponential decay of the relaxation experiments. ; save_ save_NMR_spectrometer_1 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode NMR_spectrometer_1 _NMR_spectrometer.Entry_ID 5076 _NMR_spectrometer.ID 1 _NMR_spectrometer.Manufacturer Bruker _NMR_spectrometer.Model DRX _NMR_spectrometer.Field_strength 600 save_ save_NMR_spectrometer_2 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode NMR_spectrometer_2 _NMR_spectrometer.Entry_ID 5076 _NMR_spectrometer.ID 2 _NMR_spectrometer.Manufacturer Varian _NMR_spectrometer.Model Inova _NMR_spectrometer.Field_strength 750 save_ save_NMR_spectrometer_3 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode NMR_spectrometer_3 _NMR_spectrometer.Entry_ID 5076 _NMR_spectrometer.ID 3 _NMR_spectrometer.Manufacturer Varian _NMR_spectrometer.Model Inova _NMR_spectrometer.Field_strength 500 save_